Localization of a gamma-glutamyl-transferase-related gene family on chromosome 22 |
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Authors: | Christine Morris Céline Courtay Ad Geurts van Kessel Johanna ten Hoeve Nora Heisterkamp John Groffen |
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Institution: | (1) Cytogenetic and Molecular Oncology Unit, Christchurch Hospital, Christchurch, New Zealand;(2) Unité Associée 597, Centre National de la Recherche Scientifique, 30, Rue Lionnois, F-54000 Nancy, France;(3) Department of Human Genetics, University Hospital Nijmegen, P.O. Box 9109, NL-6500 HB Nijmegen, The Netherlands;(4) Section of Molecular Diagnosis, Department of Pathology, Children's Hospital Los Angeles, 4650 Sunset Boulevard, 90027 Los Angeles, CA, USA |
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Abstract: | A gene family encompassing a minimum of four genes or pseudogenes for gamma-glutamyl transferase (GGT; EC 2.3.2.2) is present on chromosome 22q11. We have previously isolated a cDNA related to GGT but clearly not belonging to its gene family. The chromosomal location of this related gene, GGTLA1, has been determined by both isotopic and fluorescence in situ hybridization to metaphase cells and by Southern blot analysis of somatic cell hybrid DNAs. We show that GGTLA1 is part of a distinct gene family, which has at least four members (GGTLA1, GGTLA2, GGTLA3, GGTLA4). At least two loci are located on chromosome 22 within band q11 and proximal to the chronic myelogenous leukemia (CML) breakpoint in BCR (breakpoint cluster region gene). At least one other member is located more distally between the breakpoints found in Ewings sarcoma and CML. Some of the GGT and GGTLA family members are located on NotI restriction enzyme fragments of a similar size. Combined results indicate that a segment of human chromosome 22q11 has undergone largescale amplification events relatively recently in evolution. |
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