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不同温度下锐劲特对受马拉硫磷抑制的麦穗鱼AChE活性恢复的影响
引用本文:顾晓军,李少南,樊德方.不同温度下锐劲特对受马拉硫磷抑制的麦穗鱼AChE活性恢复的影响[J].生命科学研究,2000,4(4):367-373.
作者姓名:顾晓军  李少南  樊德方
作者单位:1. 福建农业大学,植保系,中国福建,福州,350002
2. 浙江大学,农药环境毒理研究所,中国浙江,杭州,310029
基金项目:Supported by Natural Science Foundation of Zhejiang Province( 392 0 4 3)
摘    要:研究了15~17℃、20~22℃下,0.01mg/L锐劲特对被1mg/L马拉硫磷抑制的麦穗鱼(Pseudorasbora parva)脑乙酰胆碱酯酶(AChE)活性恢复的影响。结果表明:在15~17℃,经48h抑制,AChE被抑制了40%,恢复144h后,处理组与无锐劲特对照都恢复到了空白对照的80%以上。在此温度下锐劲特对AChE活性恢复的影响不明显;而在20~22℃下,经相同的处理时间,AChE被抑制了70%,恢复240h后,处理组与无锐劲特对照都只恢复到了对照的70%。其间于144、192h,AChE活性差异达显著水平(P〈0.05),说明了在此温度下锐劲特对AChE活性恢复的影响。文中还讨论了恢复研究的意义。

关 键 词:乙酰胆碱酯酶  锐劲特  马拉硫磷  麦穗鱼  温度

Effect of Fipronil on Malathion-inhibited Topmouth Gudgeon (Pseudorasbora parva) Brain AChE Recovery at Different Temperatures
GU Xiao-jun,LI Shao-nan,FAN De-fang.Effect of Fipronil on Malathion-inhibited Topmouth Gudgeon (Pseudorasbora parva) Brain AChE Recovery at Different Temperatures[J].Life Science Research,2000,4(4):367-373.
Authors:GU Xiao-jun  LI Shao-nan  FAN De-fang
Abstract:Effect of 0.01 mg/L fipronil on 1 mg/L malathion-inhibited topmouth gudgeon (Pseudorasbora parva) acetylcholinesterase (AChE) recovery at 15~17 ℃ and 20~22 ℃ respectively was studied. AChE was inhibited by 40 percent in both treatment and control without fipronil after 48 h exposure and recovered to over 80 percent after 144 h recovery at 15~17 ℃. At this temperature, effect of fipronil on AChE recovery was not discernible. However, after same exposure period, AChE in both treatment and control without fipronil was inhibited by 70 percent and recovered only to 70 percent after 240 h at 20~22 ℃. The difference between treatment and control without fipronil was significant at 144 h and 192 h respectively(P<0.05), which indicated the effect of fipronil on AChE recovery at this temperature. The importance of recovery study was also discussed.
Keywords:acetylcholinesterase( ACh E)  fipronil  malathion  Pseudorasbora par
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