Enzyme-Linked Immunosorbent Assay (ELISA) of a Coryneform Isolated from Ratoon Stunted Sugarcane |
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| Authors: | D. Pillay R. A. Oellermann |
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| Affiliation: | School of Applied Sciences, M. L. Sultan Technikon, P.O. Box 1334, Durban, 4000 (South Africa).;Department of Microbiology, University of Durban-Westville, Private Bag X54001, Durban, 4000 (South Africa). |
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| Abstract: | An enzyme-linked immunosorbent assay (ELISA) was developed for the coryneform bacterium isolated from ratoon stunted surgarcane. Parameters governing the ELISA were optimized. These included antibody coating concentration, conjugate concentration and diluent, antigen diluent, the washing procedure, incubation temperature for sample and conjugate, pH and time. The level of detectability of the cultured bacterium by the ELISA was ≥ 1 × 104 Zellen · ml-1. |
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