Influence of culture modes on the microbial production of hyaluronic acid by <Emphasis Type="Italic">Streptococcus zooepidemicus</Emphasis> |
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Authors: | Long Liu Guocheng Du Jian Chen Miao Wang Jun Sun |
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Institution: | (1) School of Biotechnology, Jiangnan University, Wuxi, 214122, China;(2) Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China;(3) State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China;(4) School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China;(5) Institute of Information Technology, Jiangnan University, Wuxi, 214122, China |
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Abstract: | The principal objective of this study was to assess the effects of culture modes including batch culture, pulse fed-batch
culture, constant feeding rate fed-batch culture, and exponential fed-batch culture on the production of hyaluronic acid (HA)
by Streptococcus zooepidemicus. Batch cultures had the highest levels of HA productivity, whereas fed-batch cultures were more favorable with regard to
cell growth, and exponential fed-batch cultures evidenced the highest cell concentrations. A two-step culture model was proposed
to enhance HA production: an exponential fed-batch culture was conducted prior to 8 h and then sucrose supplementation was
applied for 8 h to start the batch fermentation of S. zooepidemicus. HA production and productivity were increased by 36 and 37% in the proposed two-step culture process as compared with that
observed in the batch culture, respectively. The proposed two-step culture model can be applied in the production of secondary
metabolites, and particularly of the exopolysaccharides. |
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Keywords: | hyaluronic acid fed-batch culture culture modes Streptococcus zooepidemicus |
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