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Stereospecificity of the hydrogen transfer catalyzed by human placental aldose reductase. |
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| Authors: | H B Feldman P A Szczepanik P Havre R J Corrall L C Yu H M Rodman B A Rosner P D Klein B R Landau |
| Abstract: | Placental aldose reductase (EC 1.1.1.21) was incubated with glucose in the presence of [4A-2H] NADPH prepared in the oxidation of [2-2H] isocitrate by isocitrate dehydrogenase (EC 1.1.1.42) or [4B-2H] NADPH prepared in the oxidation of [1-2H] glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The sorbitol formed from [4A-2H] NADPH contained deuterium and from [4B-2H] NADPH it did not. Therefore, aldose reductase in an A-type enzyme. |
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