Comparative study of Ca2+ binding to lipoprotein(a) and low density lipoprotein by spin labeling |
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Authors: | Vesna Nöthig-Laslo Günther Jürgens Anton Holasek |
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Institution: | 1. Ruder Bo?kovi? Institute, 41001 Zagreb Yugoslavia;2. Institute of Medical Biochemistry, University of Graz, A-8010 Graz Austria |
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Abstract: | The effect of Ca2+ binding on the dynamic properties of various spin labeled fatty acids in lipoprotein(a) (Lp(a)) was studied in comparison with low density lipoprotein (LDL) isolated from human plasma. In contrast to LDL, binding of Ca2+ to Lp(a) induced broadening of the lines in the ESR spectra of the spin labeled stearic acids. In 1.6 M NaBr solutions the thermotropic change in the surface structure was observed in both lipoproteins at similar temperatures. Ten millimolar concentration of Ca2+ shifted the temperature of the thermotropic change in the surface structure of Lp(a) to considerably higher values. We conclude that Ca2+ binding to Lp(a) induces changes in the lipid structure of the particle surface. |
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Keywords: | Lipoprotein(a) low density lipoprotein spin labeling thermotropic behaviour lipid structure |
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