The staining pattern of human sperm with Diff Quik: relationship with sperm head morphology and a sperm chromatin structure assay (SCSA) |
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Authors: | Moska Natalie Murray Elaine Wakefield Penelope Matson Phillip |
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Affiliation: | Hollywood Fertility Centre, Hollywood Private Hospital, Nedlands 6009, Western Australia, Australia. natalie.moska@hollywoodivf.com |
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Abstract: | The dark staining of human sperm heads by Diff Quik is significantly correlated with abnormal sperm head morphology (r=0.51, p<0.0001), but is not associated with changes in sperm chromatin detected by a sperm chromatin structure assay (SCSA; r=0.18, p>0.09). Whilst valuable in the assessment of head morphology, it is concluded that Diff Quik staining is not a useful substitute for the SCSA to assess human sperm chromatin. |
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