|
|||||
|
|
Preferential utilization of endogenous arachidonate by cyclo-oxygenase in incubations of human platelets |
|
| Authors: | L Sautebin D Caruso G Galli R Paoletti |
| Institution: | Institute of Pharmacology and Pharmacognosy, University of Milan, Via A. del Sarto 21, Milan, Italy |
| Abstract: | Thromboxane B2 (TXB2) and 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) formed from the endogenous and exogenous arachidonate during human platelet incubation, was evaluated by selected ion monitoring (SIM). TXB2 formed from endogenous substrate accounted for about one third of the total, whereas the great part of 12-HETE derived from exogenous arachidonate. These data indicate that under the tested conditions the pool of arachidonate that acts as substrate for cyclo-oxygenase is different from the pool that acts as substrate for lipoxygenase and that the arachidonate released from phospholipids is preferentially utilized by cyclo-oxygenase. |
| Keywords: | 12-HETE Arachidonic acid Cyclo-oxygenase Lipoxygenase Human platelets 12-HETE 12-hydroxy-5 8 10 14-eicosatetraenoic acid 12-HPETE 12-hydroperoxy-5 8 10 14-eicosatetraenoic acid HHT 12-hydroxy-5 8 10-heptadecatrienoic prostaglandins PRP platelet-rich plasma SIM selected ion monitoring RIA radioimmunoassay TLC thin-layer chromatography RGLC radiogaschromatography BSTFA To whom correspondence should be addressed |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |