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| 一株产红色素菌株及对其红色素结构的鉴定 | |
| 引用本文： | 张梁,郝名慧,楼志华,石贵阳.一株产红色素菌株及对其红色素结构的鉴定[J].生物加工过程,2008,6(2):28-32. |
| 作者姓名： | 张梁 郝名慧 楼志华 石贵阳 |
| 作者单位： | 1. 江南大学,工业生物技术教育部重点实验室,无锡,214036;江南大学,生物工程学院,无锡,214036 2. 江南大学,生物工程学院,无锡,214036 3. 杭州师范学院,生命科学院,杭州,310036 |
| 基金项目： | 国家高技术研究发展计划(863计划)重点课题 |
| 摘 要： | 从土壤中分离到一株产红色素的细菌H31,根据该菌株的16S rDNA序列与GenBank中已有序列比对的结果,并结合菌落形态和常规生理生化鉴定方法对该菌株进行鉴定,结果表明:该菌在细菌分类学上属于肠杆菌科,其与黏质沙雷氏菌(Serratia marcescens)同源性最高,但该菌株生理生化实验中的赖氨酸脱羧酶、鸟氨酸脱羧酶以及精氨酸双水解酶实验与报道的S. marcescens的结果不一致,为一株新的黏质沙雷氏菌S. marcescens H31。通过紫外光谱、质谱和核磁共振等方法分析,确定其产生的红色素为灵菌红素。 |
| 关 键 词： | 灵菌红素 红色素 16S rDNA 菌种鉴定 |
| 文章编号： | 1672-3678(2008)02-0028-05 |
| 修稿时间： | 2007年5月19日 |
Identification of a zymophyte strain and elucidation the structure of its red pigment |
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| ZHANG Liang,HAO Ming-hui,LOU Zhi-hua,SHI Gui-yang.Identification of a zymophyte strain and elucidation the structure of its red pigment[J].Chinese Journal of Bioprocess Engineering,2008,6(2):28-32. | |
| Authors: | ZHANG Liang HAO Ming-hui LOU Zhi-hua SHI Gui-yang |
| Institution: | ZHANG Liang1; 2; HAO Ming-hui2; LOU Zhi-hua3; SHI Gui-yang1; 2 (1.Key Laboratory of Industrial Biotechnology of the Ministry of Education; Jiangnan University; Wuxi 214036; China; 2.School of Biotechnology; 3.School of Life Sciences; Hangzhou Normal University; Hangzhou 310036; China); |
| Abstract: | A red pigment producing bacterial strain H31 was isolated from soil.The strain was identified by 16S rDNA sequence and general physiological and biochemical properites.16S rDNA sequence of the strain had the similarity of 99% with that of the Serratia marcescens and the strain was categorized to be Enterobacteria.Whereas the general physiological and biochemical properties of lysine decarboxylase,ornithine decarboxylase and arginine dihydrolase were different from that of the reported S.marcescens.It was po... |
| Keywords: | prodigiosin red pigment 16S rDNA strain identification |
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