Transfer RNA: change of conformation upon aminoacylation determined by Raman spectroscopy |
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| Authors: | G J Thomas M C Chen R C Lord P S Kotsiopoulos T R Tritton S C Mohr |
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| Institution: | Department of Chemistry, Southeastern Massachusetts University North Dartmouth, Massachusetts 02747 USA;Spectroscopy Laboratory and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 USA;Department of Chemistry, Boston University Boston, Massachusetts 02215 USA |
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| Abstract: | Laser-excited Raman spectra of yeast tRNAs in charged and uncharged states are compared to reveal differences in base stacking. Aminoacylation of unfractionated tRNA reduces considerably the amount of stacking of both adenine and pyrimidine residues. In Phe-tRNAPhe, however, only the adenine residues appear to be significantly less stacked after aminoacylation. The overall degree of order in the backbone of yeast tRNAs is little affected by these changes in base-stacked secondary structure. |
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