Cloning and nucleotide sequence of the mphB gene for macrolide 2'-phosphotransferase II in Escherichia coli |
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| Authors: | Norihisa Noguchi Jin Katayama Koji O'Hara |
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| Affiliation: | Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-03, Japan |
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| Abstract: | Abstract Macrolide 2'-phosphotransferase II [MPH(2')II] inactivates macrolide antibiotics. The mphB gene for MPH(2')II was cloned from Escherichia coli and sequenced. Analysis of the nucleotide sequence indicated that mphB encoded a protein of 302 amino acids with a molecular mass of 34483 Da. The carboxy-terminal region of the deduced protein contained a sequence that resembled a conserved functional domain in aminoglycoside phosphotransferases. |
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| Keywords: | Gene cloning Nucleotide sequence Macrolide antibiotic resistance Erythromycin Macrolide 2'-phosphotransferase II Escherichia coli |
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