Studies on the effect of the pyrethroid insecticide Decamethrin on ionic transport through the in vitro skin of Rana esculenta

The effect of the insecticide Decamethrin on ionic transport through the isolated skin of Rana esculenta was studied; the skins were bathed with 1-2 mEq Na2SO4 or choline-Cl solutions (exterior), and with Ringer normal (interior). Under open circuit (OC) conditions, mucosal Decamethrin (10(-6) M), did not provoke changes in Na+ fluxes. At 10(-5) M there was a slight inhibition of the JoNa+ after 30 min. The Cl- fluxes did not change. With longer treatments (60-90 min, OC, 10(-5) M) the JnNa+ was inhibited; at 10(-4) M it was augmented. The JnH+ was not affected. Serosal Decamethrin did not modify Na+ and H+ fluxes. In short circuit conditions, Decamethrin (10(-5) M) in the mucosal face inhibited the JnNa+; the JnH+ did not change in these conditions. The abscence of interaction of mucosal Decamethrin with Amiloride was shown.