Androgens stimulate sex change in protogynous grouper,Epinephelus coioides: spawning performance in sex-changed males |
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Authors: | Yeh Shinn-Lih Kuo Ching-Ming Ting Yun-Yuan Chang Ching-Fong |
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Institution: | Department of Aquaculture, National Taiwan Ocean University, Keelung 202, Taiwan, ROC. |
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Abstract: | We examined the efficacy of androgens (1.0 mg/kg body mass), testosterone (T), 11-ketotestosterone (11-KT), 17alpha-methyltestosterone (MT), testosterone propionate (TP) or androgen mixture (T, MT and TP in an equal ratio), for induction of sex change in protogynous orange-spotted grouper, Epinephelus coioides. The spawning performance in sex-changed males was also investigated. MT and androgen mixture at a dose of 1.0 mg/kg BW induced a sex transition and completion of spermatogenesis up to the functional male phase. The androgen mixture was most effective. Significantly, higher plasma T levels were found in MT and androgen mixture groups compared to control and other androgen implantation (T, TP or 11-KT) groups. We found that plasma levels of estradiol-17beta (E2) or 11-KT were not different among treated groups. Sex-changed males could successfully fertilize mature eggs. Fertilization and hatching rates were of 23.5-70.4% and 8.4-44.6%, respectively. The data demonstrated that induction of sex change by exogenous androgens in groups could apply to the aquaculture field for seed production. |
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Keywords: | Fertilization Gonadal development Grouper Sex change Sex steroids Reproduction Spawning Testosterone |
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