Transformation-defective v-ski induces MyoD and myogenin expression but not myotube formation. |
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Authors: | C Colmenares J K Teumer and E Stavnezer |
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Institution: | Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati Medical Center, Ohio 45267-0524. |
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Abstract: | The ski oncogene induces muscle differentiation in otherwise nonmyogenic quail embryo cells (C. Colmenares and E. Stavnezer, Cell 59:293-303, 1989). Here we report that v-ski induces both MyoD and myogenin expression, suggesting that activation of these muscle regulatory genes may be a critical step in ski-induced myogenesis. We also describe a transformation-defective mutant of v-ski (tdM5i) that fails to induce myotube formation, although it induces the expression of many muscle-specific genes, including the MyoD and myogenin genes. Therefore, if activation of MyoD and myogenin expression is a necessary component of the myogenic program triggered by ski, it is clearly insufficient to account for complete muscle differentiation. |
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