Uptake of Gibberellic Acid in Intact and Scarified Seeds of Barbarea vulgaris

Uptake of gibberellic acid as a function of duration of exposure, external concentration, and seed lot was measured in seeds of yellow rocket (Barbarea vulgaris R. Br.) by means of lettuce hypocotyl bioassays of the acidic, basic, or neutral fractions of seed extracts and by uptake of ¹⁴C-GA₃. In intact seeds, where mM levels of GA₃ promoted only 25% germination, uptake was completed within 24 h of exposure. The maximum uptake was about 0.2% of external amount. Although germination promotion by GA₃ differed among seedlots of yellow rocket, relative uptake (percentage of the external GA₃) was nearly the same. The relative rate of uptake of GA₃ was similar for scarified and intact seeds, but germination was promoted in scarified seeds by much lower levels of GA₃ than in intact seeds. Total uptake in scarified seeds was much higher, however (about 10% of the external amount). In seeds imbibed in H₂O, practically no endogenous GA-like activity was detected in either the acidic, basic, or neutral fractions. It was also apparent that intact seeds could take up quantities of GA₃ that failed to promote germination, but were comparable to quantities that promoted germination in scarified seeds.