Adsorption of DNA on clay minerals: protection against DNaseI and influence on gene transfer |
| |
Authors: | Eric Paget Lucile Jocteur Monrozier Pascal Simonet |
| |
Institution: | Laboratoire d'Ecologie Microbienne du Sol, URA CNRS 1450, UCBLyon, Villeurbanne, France |
| |
Abstract: | Abstract Numerous authors have investigated DNA relationships with sandy soil. A model composed of various DNAs adsorbed on montmorillonite clay was developed to assay enzyme (DNaseI) activity on clay-adsorbed nucleic acids. The extent of DNA adsorption was affected by the concentration and valency of the cations used (Mg2+, Ca2+, Na+), indicating a charge-dependent process. Calf thymus DNA was found to be highly adsorbed by smectite (up to 30 mg g−1 of dry clay). Adsorbed DNA was shown to be more resistant to degradation by DNaseI than free DNA. Experimental data with plasmid and short linear amplified (through polymerase chain reaction) DNA showed that protection against nucleases was only partial. Nevertheless, clay-adsorbed DNA was found to be still able, even after a strong DNaseI treatment, to artificially transform competent Escherichia coli cells. The results show that persistance of DNA and gene transfer by genetic transformation may occur in soil. |
| |
Keywords: | Plasmid DNA DNaseI GEM Adsorption isotherm Montmorillonite |
|
|