|
|||||
|
|
A high recovery method for concentrating microgram quantities of protein from large volumes of solution |
|
| Authors: | D Mahuran P Clements M Carrella P M Strasberg |
| Institution: | 2. Department of Chemical Pathology, Adelaide Children''s Hospital, North Adelaide, Australia |
| Abstract: | A method is presented for the recovery of 40-80% of the protein from a 1 microgram/ml solution. The final protein pellet is free of detergent and other ionic compounds and is thus compatible with any denaturing solution. The primary structure of the protein is unaffected by the procedure, making the final pellet an ideal sample for any analytical procedure to determine protein structure. |
| Keywords: | protein concentration protein precipitation peptide mapping microtechniques sodium dodecyl sulfate-polyacrylamide gel electrophoresis isoelectric focusing PAGE-SDS Polyacrylamide gel electrophoresis in sodium dodecyl sulfate IEF isoelectric focusing |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |