Confocal and two-photon fluorescence microscopyhave advanced the exploration of complex, three-dimensional biologicalstructures at submicron resolution. We have developed a voxel-basedthree-dimensional (3-D) imaging program (Voxx) capable of nearreal-time rendering that runs on inexpensive personal computers. Thislow-cost interactive 3-D imaging system provides a powerful tool foranalyzing complex structures in cells and tissues and encourages a morethorough exploration of complex biological image data.