Mixed-function oxygenase in juvenile rainbow trout exposed to hexachlorobenzene or 3,3',4,4'-tetrachlorobiphenyl. |
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Authors: | H Tyle M Egsmose N Harrit |
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Institution: | National Agency of Environmental Protection, Copenhagen, Denmark. |
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Abstract: | 1. 3,3',4,4'-tetrachlorobiphenyl (PCB 77), but not hexachlorobenzene, induced liver microsomal cytochrome P-450 (Cyt P-450), ethoxycoumarin-O-deethylase (ECOD) and ethoxyresorufin-O-deethylase (EROD) in rainbow trout. Maximum induction was observed in a PCB 77 injected group of fish (1.0 mg/kg, i.p. injection) 13 days after the injections being 2, 10 and 50 times the value of non-induced fish, respectively. 2. The apparent Km value of ethoxyresorufin of this induced group of fish differed only slightly from that of non-induced fish. The apparent Vmax value (EROD) was 50 times higher. 3. Freezing small pieces of liver in liquid nitrogen did not produce cytochrome P-420. 4. Fluorimetric and spectrophotometric measurements of EROD correlated. |
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