| 球形红细菌厌氧降解2,4-二硝基甲苯 |
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| 引用本文： | 梁芳楠,白红娟,柴春镜,王姗,牛伟平,赵婷婷.球形红细菌厌氧降解2,4-二硝基甲苯[J].微生物学通报,2016,43(2):279-284. |
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| 作者姓名： | 梁芳楠 白红娟 柴春镜 王姗 牛伟平 赵婷婷 |
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| 作者单位： | 1. 中北大学化工与环境学院 山西 太原 030051,1. 中北大学化工与环境学院 山西 太原 030051,2. 中北大学朔州校区化工与环境学院 山西 朔州 036000,3. 北京航天计量测试技术研究所 北京 100076,4. 山西省农药检定所 山西 太原 030001,1. 中北大学化工与环境学院 山西 太原 030051 |
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| 基金项目： | Enterprise Program Foundation of Shanxi Province (No. 20080311027-1) |
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| 摘 要： | 【目的】研究不同环境条件对2,4-二硝基甲苯(2,4-DNT)生物降解的影响。【方法】采用光合细菌球形红细菌在温度为30 °C的光照培养箱中厌氧降解2,4-DNT，并用高效液相色谱仪测定其浓度。【结果】去除2,4-DNT的最佳条件是初始浓度40 mg/L、初始pH 7.0和接种量15%。另外，2,4-DNT在菌体延滞期被细胞吸收,然后在指数期作为碳源被降解。2,4-DNT的去除率在72 h达到98.8%。从液相色谱图中观察到有2种中间代谢产物，但在120 h内产物被逐渐降解。2,4-DNT的去除动力学符合一级速率模型。【结论】不同条件下2,4-DNT的去除率表明球形红细菌能有效降解2,4-DNT。
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| 关 键 词： | 厌氧降解 2 4-DNT 动力学 球形红细菌 |
Anaerobic biodegradation of 2,4-dinitrotoluene by Rhodobacter sphaeroides |
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| LIANG Fang-Nan,BAI Hong-Juan,CHAI Chun-Jing,WANG Shan,NIU Wei-Ping and ZHAO Ting-Ting.Anaerobic biodegradation of 2,4-dinitrotoluene by Rhodobacter sphaeroides[J].Microbiology,2016,43(2):279-284. |
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| Authors: | LIANG Fang-Nan BAI Hong-Juan CHAI Chun-Jing WANG Shan NIU Wei-Ping and ZHAO Ting-Ting |
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| Institution: | 1. School of Chemical Engineering and Environment, North University of China, Taiyuan, Shanxi 030051, China,1. School of Chemical Engineering and Environment, North University of China, Taiyuan, Shanxi 030051, China,2. School of Chemical Engineering and Environment, North University of China, Shuozhou, Shanxi 036000, China,3. Beijing Aerospace Institute for Metrology and Measurement Technology, Beijing 100076, China,4. Institute for the Control of Agrochemicals, Taiyuan, Shanxi 030001, China and 1. School of Chemical Engineering and Environment, North University of China, Taiyuan, Shanxi 030051, China |
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| Abstract: | Objective] To investigate the biodegradation of 2,4-dinitrotoluene (2,4-DNT) under various environmental conditions by Rhodobacter sphaeroides. Methods] 2,4-DNT was anaerobically biodegraded under 30 °C in illumination incubator using photosynthetic bacterium Rhodobacter sphaeroides. The concentration of 2,4-DNT in liquid medium were detected by HPLC. Results] Optimum conditions for the removal of 2,4-DNT were initial concentration of 40 mg/L, initial pH 7.0 and inoculation quantity of 15%. In addition, 2,4-DNT could be absorbed by the cells in lag phase, then degraded as carbon source in exponential phase. The removal rate of 2,4-DNT achieved 98.8% at 72 h. Using HPLC, two different intermediate metabolites were also observed, however, these decreased gradually within 120 h. Furthermore, the removal kinetics of 2,4-DNT corresponded with the first-order rate model. Conclusion] The removal rate of 2,4-DNT under different conditions indicated that Rhodobacter sphaeroides is efficient in biodegrading 2,4-DNT. |
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| Keywords: | Anaerobic biodegradation 2 4-DNT Kinetics Rhodobacter sphaeroides |
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