Haemolytic reaction of Listeria monocytogenes on bilayer Columbia Agar plates with defibrinated guinea-pig blood |
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| Authors: | Dulce MT Schuch J Moore RH Madden WE Espie |
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| Institution: | Laboratório National de Referência Animal do Ministério da Agriculture e Reforma Agrária, Estrada da Ponta Grossa, 3036 Porto Alegre/RS, Brazil CEP 91700;*Department of Food Microbiology, The Queen's University of Belfast, Agriculture and Food Science Centre, Newforge Lane, Belfast BT9 5PX, UK |
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| Abstract: | Haemolysis on blood agar is an important test for the differentation of pathogenic from non-pathogenic Listeria species. The food-borne pathogen Listeria monocytogenes can only be differentiated from harmless L. innocua by its weak β-haemolysis on blood agar. This study demonstrates that L. monocytogenes , which is weakly haemolytic on sheep or horse blood agar forms clear zones of haemolysis on guinea-pig blood agar, which renders haemolysis enhancing methods, such as the CAMP test unnecessary. |
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