不同抗、感水稻品种对褐飞虱肠道菌群的影响

本研究旨在探究取食不同抗性水稻品种对褐飞虱肠道菌群多样性及丰度的影响.将生长一致的3龄褐飞虱若虫分别置于高抗水稻品种RHT、高感水稻品种TN1以及中等抗性水稻品种ZH11,取食1d和3d后,分别对褐飞虱肠道进行取样,提取总DNA并通过HiSeq 2500平台对肠道细菌16S rRNA-V4区进行测序.对测序结果进行分析...

Effects of Resistant and Sensitive Rice Varieties on Gut Microbiota of Nilaparvata lugens

This study aims to investigate the effects of resistant and sensitive rice varieties on the diversity and abundance of the gut microbiota of brown planthopper(BPH).The 3rd instar BPH nymphs were feeding on resistant rice variety RHT,susceptible rice variety TN1,and medium-resistant rice variety ZH11,and the intestines of BPH were sampled after 1 and 3 d.Total DNA were extracted and sequenced through the HiSeq 2500 platform for 16S rRNA-V4 region of bacteria in the gut.The sequencing results were analyzed to count the number of OTUs(Operational taxonomic unit)in different samples.The species composition,abundance,and the alpha diversity were counted at different levels of taxonomies.The specific and common bacteria of BPH after feeding with three different rice varieties were counted.The bacteria with high abundance and significant difference were analyzed.More than 70000 valid sequences were obtained from each sample,and a certain number of OTUs were clustered based on sequence similarity.There were 213 common OTUs and 424,77,and 130 unique OTUs in 1 d samples of BPH after feeding on RHT,TN1,and ZH11,respectively,and 217 common OTUs and 140,162,63 unique OTUs in 3 d samples.The Acinetobacter and Wolbachia were two dominant gut microbiota species,and the content of Acinetobacter in the brown planthopper that fed on RHT was lower than that fed on TN1 and ZH11.Among 319 genera of bacteria in BPH,8 types of bacteria demonstrated significant differences in BPH after feeding on RHT,TN1 and ZH11 rice,respectively,which may be closely related to the BPH feeding on different resistant rice varieties.BPH can respond to different resistant rice varieties by changing the intestinal bacterial composition.This study lays a foundation for further exploring the intestinal microbiota of the BPH and their response to different rice varieties,and also provides a reference for controlling brown planthoppers using microbial resources.