| 黑曲霉单宁酶基因Tan2克隆与表达 |
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| 引用本文： | 李红叶,陈立佼,刘明丽,郭天杰,王道平,潘映红,赵明.黑曲霉单宁酶基因Tan2克隆与表达[J].生物技术通报,2021(3):44-52. |
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| 作者姓名： | 李红叶 陈立佼 刘明丽 郭天杰 王道平 潘映红 赵明 |
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| 作者单位： | 云南农业大学龙润普洱茶学院;中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程;云南农业大学云南省药用植物生物学重点实验室云南农业大学西南中药材种质创新与利用国家地方联合工程研究中心 |
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| 基金项目： | 国家自然科学基金项目(31760225);云南省中青年学术技术带头人后备人才培养项目(2017HB026)。 |
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| 摘 要： | 单宁酶(Tannase,EC 3.1.1.20)能水解单宁中的酯键和羧酚酸键,产生没食子酸以及对应醇,在食品、饮料、饲料、制药、医药、化妆品等各类工业中应用广泛,也在普洱茶发酵中具有重要作用.从普洱茶发酵中分离的黑曲霉菌株PU001中克隆得到单宁酶基因Tan2,并连接到表达载体pCold-Ⅰ构建BL21-pCdd Ⅰ原...
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| 关 键 词： | 单宁酶 黑曲霉 普洱茶 |
Cloning and Expression of Tannase Gene Tan2 from Aspergillus niger |
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| LI Hong-ye,CHEN Li-jiao,LIU Ming-li,GUO Tian-jie,WANG Dao-ping,PAN Ying-hong,ZHAO Ming.Cloning and Expression of Tannase Gene Tan2 from Aspergillus niger[J].Biotechnology Bulletin,2021(3):44-52. |
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| Authors: | LI Hong-ye CHEN Li-jiao LIU Ming-li GUO Tian-jie WANG Dao-ping PAN Ying-hong ZHAO Ming |
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| Institution: | (College of Longrun Pu-erh Tea,Yunnan Agricultural University,Kunming 650201;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081;The Key Laboratory of Medicinal Plant Biology of Yunnan Province/National&Local Joint Engineering Research Center on Germplasm Innovation&Utilization of Chinese Medicinal Materials in Southwestern China,Yunnan Agricultural University,Kunming 650201) |
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| Abstract: | Tannase(EC 3.1.1.20)can hydrolyze the ester bond and carboxyphenolic acid bond in tannins to produce gallic acid and corresponding alcohols.It is widely used in food,beverage,feed,pharmacy,medicine,cosmetics and other industries,and also plays an important role in the fermentation of Pu-erh tea.In this work,tannase gene Tan 2 was cloned from Aspergillus niger PU001,which was previously isolated from fermentation in Pu-erh tea.It was ligated with expression vector pCold-Ⅰ to construct the prokaryotic cold inducible expression system of BL21-pCold Ⅰ,which was transformed into Escherichia coli BL21.SDS-PAGE and mass spectrometry identification showed that tannase Tan 2 was expressed successfully.This result provides a basis for further survey on the role of tannase in the fermentation of Pu-erh tea and application in other fields. |
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| Keywords: | tannase Aspergillus niger Pu-erh tea |
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