| 解淀粉芽胞杆菌HR-2的分离鉴定及对水稻稻瘟病菌的拮抗效果 |
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| 引用本文： | 李瑾,彭可为,潘求一,朱哲远,彭迪. 解淀粉芽胞杆菌HR-2的分离鉴定及对水稻稻瘟病菌的拮抗效果[J]. 生物技术通报, 2021, 0(3): 27-34 |
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| 作者姓名： | 李瑾 彭可为 潘求一 朱哲远 彭迪 |
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| 作者单位： | 湖南大学研究生院隆平分院;湖南省农业科学院/农业生物技术研究所;湖南农业大学植物保护学院;湖南省安乡县植保植检站 |
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| 基金项目： | 国家自然科学基金项目(31501601);湖南省农业科学院创新资金(2019LS05)。 |
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| 摘 要： | 稻瘟病是一种严重威胁全球粮食安全的水稻真菌病害.本研究采用平板对峙法从湖南岳阳地区筛选出1株对水稻稻瘟病菌具有高效拮抗活性的菌株HR-2.通过形态特征验证、16S rRNA、gyrA和tuf基因序列比对分析,鉴定该菌株为解淀粉芽胞杆菌(Bacillus amyloliquefaciens).抑菌广谱性测定结果表明菌株H...
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| 关 键 词： | 水稻稻瘟病 解淀粉芽胞杆菌 分离鉴定 生物防治 |
Isolation and Identification of Bacillus amyloliquefaciens HR-2 and Biological Control of Rice Blast |
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| LI Jin,PENG Ke-wei,PAN Qiu-yi,ZHU Zhe-yuan,PENG Di. Isolation and Identification of Bacillus amyloliquefaciens HR-2 and Biological Control of Rice Blast[J]. Biotechnology Bulletin, 2021, 0(3): 27-34 |
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| Authors: | LI Jin PENG Ke-wei PAN Qiu-yi ZHU Zhe-yuan PENG Di |
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| Affiliation: | (Longping Branch,Graduate School of Hunan University,Changsha 410125;Institute of Agricultural Biotechnology,Hunan Academy of Agriculture Sciences,Changsha 410125;Institute of Plant Protection of Hunan Agricultural University,Changsha 410128;Anxiang County Plant Protection Station of Human Province,Anxiang 415600) |
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| Abstract: | Magnaporthe oryzae is a rice fungal disease that seriously threatens global food security.A strain HR-2 with high antagonistic activity against M.oryzae was screened from Yueyang district,Hunan province by plate confrontation method in this study.The strain HR-2 was identified as Bacillus amyloliquefaciens by morphological verification,16S rRNA,gyrA and tuf gene sequence analysis.The results of broadspectrum inhibition showed that the strain HR-2 had a fine inhibitory effect on M.oryzae,Sclerotinia sclerotiorum and Ralstonia solanacearum,and the inhibition rates were 82.34%,72.32%and 72.64%,respectively.The results of pot experiment demonstrated that the control effects of HR-210-times diluted solution and 100-times diluted solution on M.oryzae were 63.81%and 40.77%respectively,among which the control effects of HR-210-times diluted solution were similar to that of 50 mg/L azoxystrobin.The above results indicated that B.amyloliquefaciens HR-2 had a wide antibacterial spectrum and an obvious control effect on M.oryzae.Our research lays a foundation for the development of biological control agent against M.oryzae. |
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| Keywords: | Magnaporthe oryzae Bacillus amyloliquefaciens isolated and identification biological control |
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