大肠杆菌高效合成L-苯甘氨酸的研究进展

自然界存在着多种氨基酸,除用于蛋白质合成的20种外,大量用于合成具有生物活性的物质,广泛应用于食品、医药等多个领域.其中,非天然芳香族氨基酸L-苯甘氨酸作为一种重要的组成单元广泛的应用于盘尼西林、维吉霉素S、原始霉素Ⅰ等β-内酰胺类抗生素的生物合成当中.目前L苯甘氨酸主要通过化学法合成,但该方法合成收率低、污染大,且不...

Research Progress on the Efficient Synthesis of Phenylglycine by Escherichia coli

There are many kinds of amino acids in nature.In addition to the 20 kinds used in protein synthesis,a large number of amino acids are used to synthesize biologically active substances,which are widely used in food,medicine and other fields.Among them,the unnatural aromatic amino acid L-phenylglycine as an important component is widely used in the biosynthesis of β-lactam antibiotics such as penicillin,virginomycin S,and protomycin I.At present,L-phenylglycine is mainly synthesized by chemical methods,but this method has low synthesis yield,high pollution,and it is not easy to obtain monochiral compounds.Biosynthesis of L-phenylglycine has the advantages of mild reaction conditions and good product stereoselectivity,so it has received extensive attention.Through the analysis of the two biosynthetic pathways of L-phenylglycine,the screening of relevant enzymes required for synthesis and the balance regeneration of cofactors,etc.,A synthetic route with acetophenone acid,mandelic acid and L-phenylalanine as substrates was gradually formed.This article mainly reviews the biosynthetic pathways and biosynthetic strategies of L-phenylglycine,and provides researchers with optimization directions,with a view to providing a theoretical reference for efficient industrial biosynthesis of L-phenylglycine.