油葵HaGPAT1基因的克隆及表达分析

该研究利用RT-PCR技术,从油葵(Helianthus annuus L.)种子中克隆了甘油-3-磷酸酰基转移酶(GPAT)基因(HaGPAT1),对其进行生物信息学分析,并通过实时荧光定量PCR技术(qRT-PCR)检测该基因在不同组织、种子不同发育时期以及不同胁迫条件下的表达特征。结果表明:HaGPAT1基因全长为1 656bp,编码551个氨基酸,相对分子量为62.132kD,等电点为8.84。系统进化树分析表明,HaGPAT1蛋白与高等植物莴苣的GPAT1亲缘关系最近。qRT-PCR分析表明,HaGPAT1基因在油葵花蕊中的表达水平最高,开花后17d的种子中次之;在干旱和盐胁迫条件下,HaGPAT1基因的表达水平均显著上调。研究推测,HaGAT1基因可能在油葵花器官发育中发挥重要作用,并且参与了油葵对干旱和高盐的抗性调节。

Cloning and Expression Analysis of HaGPAT1 Gene from Helianthus annuus L.

In this study, a glycerol 3 phosphate acyltransferase gene, named HaGPAT1, was isolated from oil sunflower seeds. The length of HaGPAT1 is 1 656 bp, encoding 551 amino acids with calculated molecular mass of 62.132 kD and isoelectric point (PI) 8.84. The expression profile of HaGPAT1 was detected by quantitative real time PCR (qRT PCR) in different tissues through different developmental stages of seeds and under abiotic stress treatments. Multiple sequences alignment showed that HaGPAT1 was closely related to GPAT1 protein of higher plants. The expression of HaGPAT1 reached its highest level in buds and up regulated under drought and salt stresses. The results suggested that HaGAT1 might play an important role in floral organ development and may be involved in abiotic stress regulation of sunflower.