QTL analysis of agronomic traits in barley based on the doubled haploid progeny of two elite North American varieties representing different germplasm groups |
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Authors: | L A Marquez-Cedillo P M Hayes A Kleinhofs W G Legge B G Rossnagel K Sato S E Ullrich D M Wesenberg |
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Institution: | (1) Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331, USA, US;(2) Department of Crop and Soil Science, Washington State University, Pullman, WA 99164, USA, US;(3) Agriculture and Agri-Food Canada, Brandon MB R7A 5Y3, Canada, CA;(4) Crop Development Centre, University of Saskatchewan, Saskatoon, Sask., S7N 5A8, Canada, CA;(5) Research Institute for Bioresources, Okayama University, Kurashiki 710-0046, Japan, JP;(6) National Small Grains Germplasm Research Facility, P.O. Box 307, Aberdeen, ID 83210, USA, US |
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Abstract: | A better understanding of the genetics of complex traits, such as yield, may be achieved by using molecular tools. This study
was conducted to estimate the number, genome location, effect and allele phase of QTLs determining agronomic traits in the
two North American malting barley (Hordeum vulgare L.) quality variety standards. Using a doubled haploid population of 140 lines from the cross of two-rowed Harrington×six-rowed
Morex, agronomic phenotypic data sets from nine environments, and a 107-marker linkage map, we performed QTL analyses using
simple interval mapping and simplified composite interval mapping procedures. Thirty-five QTLs were associated, either across
environments or in individual environments, with five grain and agronomic traits (yield, kernel plumpness, test weight, heading
date, and plant height). Significant QTL×environment interaction was detected for all traits. These interactions resulted
from both changes in the magnitude of response and changes in the sign of the allelic effect. QTLs for multiple traits were
coincident. The vrs1 locus on chromosome 2 (2H), which determines inflorescence row type, was coincident with the largest-effect QTL determining
four traits (yield, kernel plumpness, test weight, and plant height). QTL analyses were also conducted separately for each
sub-population (six-rowed and two-rowed). Seven new QTLs were detected in the sub-populations. Positive transgressive segregants
were found for all traits, but they were more prevalent in the six-rowed sub-population.QTL analysis should be useful for
identifying candidate genes and introgressing favorable alleles between germplasm groups.
Received: 18 August 2000 / Accepted: 15 December 2000 |
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Keywords: | Hordeum vulgare Two-rowed Six-rowed Agronomic traits Quantitative trait loci |
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