大鼠子宫宫腔粘连模型的建立及评价指标

子宫内膜损伤是导致宫腔粘连的最主要原因,建立有效的子宫内膜损伤动物模型是研究此类疾病发生、发展和治疗反应等不可或缺的支撑条件。通过宫腔注射95%的乙醇制造大鼠(Rattus norvegicus)子宫内膜损伤模型,检测胚胎植入数目来分析子宫内膜损伤对大鼠生育情况的影响,观察大鼠子宫内膜厚度、腺体数量和纤维化面积,分析乙醇处理对子宫内膜的影响;检测细胞角蛋白(CK-19)和波性蛋白(Vimentin)的表达水平,分析上皮细胞和间质细胞的增殖分化及子宫内膜损伤程度。结果显示,正常组大鼠子宫比损伤组更为平滑且有韧性,与正常组相比,损伤组大鼠生育率显著降低(P0.01),子宫内膜厚度变薄(P0.01)、腺体数量显著减少(P0.01),纤维化面积显著增大(P0.01),CK-19和Vimentin表达量显著下调。结果提示已成功建立大鼠子宫内膜损伤动物模型。

Establishment and Evaluation of Rat Model with Intrauterine Adhesions

Abstract: Endometrial lesions is the main reason leading to cervical adhesions. Establishing an effective animal model of endometrial injury is an indispensable condition to study the disease occurrence, development and treatment. We established a rat (Rattus norvegicus) model of endometrial injury by intrauterine injection of 95% alcohol. The thickness of endometrium, glands numbers and the area of fibrosis were observed to analyze the effects of ethanol treatment on the endometrium. The expression of cytokeratin 19 (CK-19) and Vimentin were detected to analyze the status of epithelium and mesenchymal cell. The numbers of embryo implantation were detected to analyze the effect of endometrial injury on the fertility. The results showed that the specimen of rat uterus in normal group was smooth and strong toughness compared with injury group ( Fig.1). Rat endometrium in the injury group was much thinner than that in control group (P < 0.01) ( Fig.2c). Moreover, compared with control group, the glands numbers in the injury group was significantly reduced (P < 0.01) ( Fig.2d), the area of fibrosis was observably increased (P < 0.01) ( Fig.3) and the expression of CK-19 ( Fig.4) and vimentin ( Fig.5) was remarkably decreased. What''s more, the fertility in the injury group was significantly lower than that in control group (P < 0.01) ( Fig.6). The results suggest that the animal model of rat endometrial injury is successfully established.