Two binding sites in acetylcholine receptor from Torpedo marmorata electroplax |
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Authors: | R D O'Brien R E Gibson |
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Institution: | Section of Neurobiology and Behavior, Cornell University, Ithaca, New York 14850 U.S.A. |
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Abstract: | The sensitivity of acetylcholine receptor to eleven cholinergic drugs, phospholipase A, heat and pH provided evidence that the so-called high-affinity binding (Kd for acetylcholine 11 nm in 1% Triton) and low-affinity binding (Kd 562 nm) were related to two distinct binding sites. The low-affinity binding site was less sensitive to heat and several of the cholinergic drugs, but was a little more sensitive to bungarotoxin than the high-affinity site. Zinc (0.4 mm) and EDTA (10 mm) abolished acetylcholine binding to both sites; the EDTA inhibition was time-dependent. |
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