梅毒螺旋体重组抗原酶联免疫吸附试验的建立及应用

建立梅毒螺旋体重组抗原酶联免疫吸附试验（ELISA），用于梅素血清学诊断和调查。其法，用表达的重组抗原IPN17和TmpA，建立检测血清特异抗体的间接ELISA，并与其它检测方法比较，分别检测梅毒参比血清、病人及献血员血清。其结果，敏感性、特异性均为100％。新建ELISA与TPHA的总符率为95．7％，明显高于RPR与TPHA的总符合率（89．1％）。献血员人群抗体阳性率为0．3％－0．69％，健康人群中抗体阳性率较低。

Development andApplication of an Enzyme-linked Immunoassay for Serodiagnosis of Syphilis by RecombinantTrepnoma Palidum Antigens

Objective；To develop an by Enzyme-linked Immu nosorbent Assay (ELISA) by using specific genetic Treponema pallidum recombinant antigen ( TPN17 & TmpA) and evaluate its potential use in the serodiagnosis of Syphilis. Methods The expressed and purified specific antigen were coated microtiter plates, and esta blished the ELISA. The sensitivity, specificity and positive rates of the assay were determined by detecting standard reference syphilis sera, or the sera from patients and blood donors. In addition, we also investigated the positive rates in different population of the country. Results The sensitivity and specificity of the ELISA were both 100%. The identity of the ELISA and TPHA was significantl y higher(95.7%) than that of the RPR and TPHA(89.1%). The positive rate in the s era of the blood donors was 0.3%～0.69%, and it was much lower in normal sera.