Simultaneous nitrification and denitrification by diverse <Emphasis Type="Italic">Diaphorobacter</Emphasis> sp. |
| |
Authors: | Anshuman A Khardenavis Atya Kapley Hemant J Purohit |
| |
Institution: | (1) Environmental Genomics Unit, National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur, 440 020, India |
| |
Abstract: | Eight bacterial isolates closely related to Diaphorobacter sp. were isolated from activated biomass surviving on wastewater laden with dyes and nitro-substituted chemicals and were
identified by 16S rDNA sequence analysis. The isolates showed sequence similarity of 99–100% to other Diaphorobacter strains such as ZY 2006b, F2, NA5, PCA039, D. nitroreducens KSP4, and KSP3 and 98–99% sequence homology to D. nitroreducens NA10B (type strain JCM 11421). Neighbor-joining tree revealed that all the eight strains formed tight cluster together and
also showed close clustering with other Diaphorobacter strains. Isolates demonstrated the ability to perform simultaneous nitrification and denitrification under aerobic conditions.
Strains HPC 805, 815, 821, and 856 gave highest chemical oxygen demand removal (85–93%) and ammonia removal (92–96%), which
correlated well with higher growth rates of the cultures. Simultaneously, complete removal of nitrate supplied in the medium
in presence of ammonium and acetate (electron donor) was observed in addition to aerobic nitrite release from ammonium. Thus,
the above strains showed ability to perform partial nitrification followed by further aerobic removal of common intermediate
nitrite, which indicated their potential application in treatment systems for treatment of high-nitrogen-containing wastewaters. |
| |
Keywords: | Diaphorobacter nitroreducens Simultaneous nitrification denitrification Ammonia-oxidizing bacteria |
本文献已被 PubMed SpringerLink 等数据库收录! |
|