Proneural bHLH neurogenin 2 differentially regulates Nurr1-induced dopamine neuron differentiation in rat and mouse neural precursor cells in vitro |
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Authors: | Park Chang-Hwan Kang Jin Sun Yoon Eun-Hye Shim Jae-Won Suh-Kim Haeyoung Lee Sang-Hun |
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Affiliation: | Department of Microbiology, College of Medicine, Hanyang University, Sungdong-Gu, Seoul, Republic of Korea. |
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Abstract: | Roles of Nurr1 and neurogenin 2 (Ngn2) have been shown in midbrain dopamine (DA) neuron development. We present here rat and mouse species-dependent differences of Nurr1 and Ngn2 actions in DA neuron differentiation. Nurr1 exogene expression caused an efficient generation of tyrosine hydroxylase (TH)-positive DA cells from rat neural precursor cells (NPCs). Nurr1-induced TH+ cell yields were low and highly variable depending on the origins of NPCs in mouse cultures. Coexpression of Ngn2 repressed Nurr1-induced generation of TH+ cells in rat cultures. In clear contrast, a robust enhancement in Nurr1-induced DA cell yields was observed in mouse NPCs by Ngn2. These findings imply that DA neurons may develop differently in the midbrains of these two species. |
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Keywords: | Dopaminergic differentiation Neural precursor cells Neurogenin 2 Species-dependent differences Nurr1 |
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