Purification and characterization of distinct type of mannose-sensitive fimbriae from Salmonella typhimurium |
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Authors: | Sujata Ghosh Amrisha Mittal Nirmal Kumar Ganguly |
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Institution: | Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh 160 012, India |
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Abstract: | Abstract The fimbriae (E4) of a virulent strain of Salmonella typhimurium were purified by ion exchange chromatography in an FPLC system. They had a channelled appearance under transmission electron microscope and showed a major structural subunit of 17-kDa on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The purified fimbriae were found to agglutinate guinea pig erythrocytes, but this effect was inhibited in presence of D-mannose. Immune sera raised against the Mono-Q purified fimbriae (E4) showed cross-reactivity with the type-1 fimbriae (F1) composed of 21-kDa fimbrin subunit, purified by a different method from the same strain. |
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Keywords: | Salmonella typhimurium Fimbriae Hemagglutination |
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