|
|||||
|
|
Analysis of functional coupling: mitochondrial creatine kinase and adenine nucleotide translocase |
|
| Authors: | Vendelin Marko Lemba Maris Saks Valdur A |
| Affiliation: | * Laboratory of Fundamental and Applied Bioenergetics, Institut National de la Santé et de la Recherche Médicale E0221, Joseph Fourier University, Grenoble, France † Institute of Cybernetics, Tallinn Technical University, Tallinn, Estonia ‡ Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia |
| Abstract: | The mechanism of functional coupling between mitochondrial creatine kinase (MiCK) and adenine nucleotide translocase (ANT) in isolated heart mitochondria is analyzed. Two alternative mechanisms are studied: 1), dynamic compartmentation of ATP and ADP, which assumes the differences in concentrations of the substrates between intermembrane space and surrounding solution due to some diffusion restriction and 2), direct transfer of the substrates between MiCK and ANT. The mathematical models based on these possible mechanisms were composed and simulation results were compared with the available experimental data. The first model, based on a dynamic compartmentation mechanism, was not sufficient to reproduce the measured values of apparent dissociation constants of MiCK reaction coupled to oxidative phosphorylation. The second model, which assumes the direct transfer of substrates between MiCK and ANT, is shown to be in good agreement with experiments—i.e., the second model reproduced the measured constants and the estimated ADP flux, entering mitochondria after the MiCK reaction. This model is thermodynamically consistent, utilizing the free energy profiles of reactions. The analysis revealed the minimal changes in the free energy profile of the MiCK-ANT interaction required to reproduce the experimental data. A possible free energy profile of the coupled MiCK-ANT system is presented. |
| Keywords: | |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |