| Surfactin对人乳腺癌MCF-7细胞增殖、凋亡及细胞骨架的影响 |
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| 引用本文： | 曹小红,王爱华,王春玲,鲁梅芳,焦润芝,朱慧,赵思思. Surfactin对人乳腺癌MCF-7细胞增殖、凋亡及细胞骨架的影响[J]. 生物工程学报, 2009, 25(11): 1705-1710 |
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| 作者姓名： | 曹小红 王爱华 王春玲 鲁梅芳 焦润芝 朱慧 赵思思 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院,天津,300457 |
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| 基金项目： | 国家高技术研究发展计划(863计划)(No.2007AA10Z319)资助~~ |
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| 摘 要： | 以体外培养的人乳腺癌细胞株MCF-7为研究对象,探讨surfactin对肿瘤细胞增殖、凋亡及细胞骨架的影响。MTT实验表明,surfactin能抑制MCF-7的增殖,并且呈现出浓度和时间的依赖关系,作用48h时的IC50是27.3μmol/L。AO/EB荧光染色法及流式细胞术检测发现,surfactin可诱导MCF-7发生典型的凋亡形态学改变和G2/M期阻滞。免疫荧光和免疫印迹结果表明,surfactin显著抑制了细胞内vimentin的表达,诱导了α-tubulin的解聚和重排,使细胞的骨架系统发生了剧烈的变化。可见,surfactin具有抑制MCF-7细胞增殖,诱导细胞凋亡的作用,其机制可能与细胞骨架蛋白的表达水平有关。
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| 关 键 词： | surfactin 增殖 凋亡 细胞骨架 |
| 收稿时间： | 2009-07-21 |
Effects of surfactin on proliferation, apoptosis and cytoskeleton in human breast cancer MCF-7 cells |
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| Xiaohong Cao,Aihua Wang,Chunling Wang,Meifang Lu,Runzhi Jiao,Hui Zhu and Sisi Zhao. Effects of surfactin on proliferation, apoptosis and cytoskeleton in human breast cancer MCF-7 cells[J]. Chinese journal of biotechnology, 2009, 25(11): 1705-1710 |
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| Authors: | Xiaohong Cao Aihua Wang Chunling Wang Meifang Lu Runzhi Jiao Hui Zhu Sisi Zhao |
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| Affiliation: | College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China |
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| Abstract: | We studied the effect of surfactin on cell proliferation, apoptosis and the cytoskeleton in human breast cancer cell line MCF-7 in vitro.The result of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) showed that the surfactin inhibited proliferation of MCF-7 cells in a dose- and time-dependent manner, with IC_(50) at 48 h of 27.3 μmol/L.Surfactin-induced cell death was considered to be apoptotic by observing the typical apoptotic morphological changes by AO/EB staining.Flow cytometric analysis also demonstrated that surfactin caused time-dependent apoptosis of MCF-7 cells through cell arrest at G_2/M phase.Immunofluorescence and Western blotting showed that surfactin significantly suppressed the expression of vimentin, induced the α-tubulin depolymerization and rearrangement and then the skeleton system of the cells changed dramatically.Based on our findings, surfactin can significantly inhibit the growth of MCF-7 cells and induce apoptosis. |
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| Keywords: | surfactin proliferation apoptosis cytoskeleton |
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