In vitro propagation of Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae) |
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Authors: | G. Bringmann H. Rischer J. Schlauer L. Aké Assi |
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Affiliation: | 1. Institut für Organische Chemie der Universit?t, Am Hubland, D-97074, Würzburg, Germany 2. Centre National de Floristique, Université d'Abidjan, 08 B.P. 172, Abidjan 08, Ivory Coast
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Abstract: | Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae), a West African liana producing naphthylisoquinoline alkaloids, was successfully raised from seeds in vitro. Clonal propagation was best achieved growing nodal stem segments on 1/5 Linsmaier and Skoog medium with full strength organics and supplemented with 0.02 μM thidiazuron, 4.44 μM 6-benzylaminopurine and 0.05 μM 1-naphthaleneacetic acid. Detached axillary shoots were grown on Anderson's Rhododendron medium devoid of phytohormones and rooted within one month when dipped in 4.92 μM indole-3-butyric acid. Rooted plants became acclimatized to nonsterile greenhouse conditions. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | liana micropropagation naphthylisoquinoline alkaloids tissue culture |
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