Production of Trichoderma strains with pesticide-polyresistance by mutagenesis and protoplast fusion |
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Authors: | Lóránt Hatvani László Manczinger László Kredics András Szekeres Zsuzsanna Antal Csaba Vágvölgyi |
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Institution: | (1) Department of Microbiology, University of Szeged, PO Box 533, H-6701 Szeged, Hungary;(2) Microbiological Research Group, Hungarian Academy of Sciences and University of Szeged, PO Box 533, H-6701 Szeged, Hungary |
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Abstract: | The sensitivity of two cold-tolerant Trichoderma strains belonging to the species T. harzianum and T.␣atroviride was determined to a series of pesticides widely used in agriculture. From the 16 pesticides tested, seven fungicides: copper sulfate, carbendazim, mancozeb, tebuconazole, imazalil, captan and thiram inhibited colony growth of the test strains significantly with minimal inhibitory concentrations of 300, 0.4, 50, 100, 100, 100 and 50 g/ml, respectively. Mutants resistant to carbendazim and tebuconazole were produced from both wild type strains by means of UV-mutagenesis. The cross-resistance capabilities and in␣vitro antagonistic properties of the mutants were determined. Carbendazim-resistant mutants showed total cross-resistance to benomyl and thiabendazole at a concentration of 20 g/ml. Intraspecific protoplast fusion was carried out between carbendazim- and tebuconazole-resistant mutants of both parental strains, and putative haploid recombinants with stable resistance to both pesticides were produced in the case of T.␣atroviride. These pesticide-polyresistant progenies are potential candidates for application in an integrated pest management system.This work was presented as an oral lecture in section ‘Agriculture, Soil, Forest Microbiology’ at the BioMicroWorld2005 conference. |
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Keywords: | Biocontrol agents Mutagenesis Pesticide polyresistance Protoplast fusion Trichoderma |
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