The dependence of protein release from Bacillus amyloliquefaciens on the growth phase in batch culture

For the recovery of intracellular material from bacteria it is often necessary to disrupt the cells. Much work has been done on the kinetics of protein release in beadmills,(1) homogenizers,(2) and by ultrasonication.(3) In this paper we report how the growth phase of Bacillus amyloliquefaciens grown in batch culture affects the rate of protein release by ball milling, ultrasonication, and autolysis. We further suggest that autolysis is a feasible method for disrupting Bacillus.