First evidence of Wolbachia infection in populations of grasshopper Podisma sapporensis (Orthoptera: Acrididae) |
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| Authors: | Alexander G. Bugrov Yury Yu. Ilinsky Anton Strunov Mariya Zhukova Elena Kiseleva Shin‐ichi Akimoto Haruki Tatsuta |
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| Affiliation: | 1. Institute of Systematics and Ecology of Animals, Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russia;2. Novosibirsk State University, Novosibirsk, Russia;3. Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russia;4. Institute of Chemistry and Biology, Immanuel Kant Baltic Federal University, Kaliningrad, Russia;5. Systematic Entomology, Department of Ecology and Systematics, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan;6. Department of Ecology and Environmental Sciences, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan |
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| Abstract: | The brachypterous grasshopper Podisma sapporensis (Orthoptera: Acrididae) is distributed throughout the Sakhalin, Kunashir and Hokkaido Islands. Karyotypes of this species consist of two major chromosomal races with different sex chromosome systems, XO/XX and XY/XX. Molecular phylogeographic analysis of the chromosome races and subraces confirms the genetic divergence of the races and subraces in P. sapporensis. Here we first report that P. sapporensis is infected with Wolbachia consisting of three variants on wsp locus, while gatB locus was monomorphic. Furthermore, observation of cell tissue of P. sapporensis using electron microscopy confirmed the infection of Wolbachia that was inferred from polymerase chain reaction and revealed the distribution of the bacteria in the head, thorax and abdomen of P. sapporensis embryos. Our finding may shed new light on Wolbachia as a possible agent causing hybrid dysfunction resulting from experimental crosses between chromosome races or subraces of P. sapporensis. |
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| Keywords: | chromosomal race electron microscopy gatB gene wsp gene |
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