Identification and Characterization of Novel Antibacterial Peptides from Skin Secretions of Euphlyctis cyanophlyctis |
| |
| Authors: | Ahmad Asoodeh Adel Ghorani Azam JamshidKhan Chamani |
| |
| Institution: | (1) Biomolecules Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran;(2) Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran;(3) Department of Biology, Faculty of Sciences, Mashhad-Branch, Islamic Azad University, Mashhad, Iran |
| |
| Abstract: | In this study, we extracted and purified antimicrobial peptides (AMPs) secreted from skin of Euphlyctis cyanophlyctis using reverse phase-high performance liquid chromatography. Three AMPs were isolated from skin secretions of this frog and
sequenced using tandem mass spectrometry. The purified peptides were named buforin-EC (1875.05 ± 0.5 Da), cyanophlyctin (2347.50 ± 0.5 Da)
and temporin-ECa (1013.33 ± 0.5 Da). Multiple alignments and homology search showed that buforin-EC, cyanophlyctin and temporin-ECa
had a homology of 71.43, 47.1, and 69.23% to buforin II, brevinin-2EC, and temporin-1CSc, respectively. Antimicrobial tests
demonstrated that our peptides have a great antimicrobial effect on both gram-positive and gram-negative bacteria. The results
indicated that they have an overall minimum inhibitory concentration (MIC) below 13 μM against E. coli. No hemolysis was observed in around of their MIC values. In conclusion, skin secretions of E. cyanophlyctis contain a novel class of AMPs with the proper characteristics. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
