Expression of progesterone receptor membrane component (PGRMC) 1 and 2, serpine mRNA binding protein 1 (SERBP1) and nuclear progesterone receptor (PGR) in the bovine endometrium during the estrous cycle and the first trimester of pregnancy |
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| Authors: | Magdalena K Kowalik Dominika Slonina Robert Rekawiecki Jan Kotwica |
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| Institution: | 1. Chapingo Autonomous University, Regional Universitary Unit on Arid Lands, Bermejillo, Durango, Mexico;2. Baja California Autonomous University – ICA, Mexicali, Baja California Norte, Mexico;3. Antonio Narro Autonomous Agricultural University, Torreon, Coahuila, Mexico;1. Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, Poland;2. Institute of Dendrology, Polish Academy of Sciences, Kórnik, Poland;1. General Directory for Ordination and Inspection, Council of Health, Community of Madrid, Madrid, Spain;2. Department of Animal Reproduction, INIA, Avda Puerta de Hierro s/n., Madrid, Spain;3. Department of Toxicology and Pharmacology, Faculty of Veterinary, Complutense University of Madrid, Madrid, Spain;1. Breast Cancer Prevention Center, University of Kansas Medical Center, Kansas City, KS 66160, United States;2. Department of Internal Medicine, University of Kansas Medical Center, Kansas City, KS 66160, United States;3. Department of Radiation Oncology, University of Kansas Medical Center, Kansas City, KS 66160, United States |
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| Abstract: | Progesterone (P4) is involved in the regulation of essential reproductive functions affecting the target cells through both nuclear progesterone receptors (PGRs) and membrane progesterone receptors. The aim of this study was to determine the mRNA and protein expression for PGRMC1, PGRMC2, SERBP1 and PGR within the bovine endometrium during the estrous cycle and the first trimester of pregnancy. There were no changes in PGRMC1 and PGRMC2 mRNA and protein expression during the estrous cycle, however, mRNA levels of PGRMC1 and PGRMC2 were increased (P < 0.001) in pregnant animals. SERBP1 mRNA expression was increased (P < 0.05), while the level of this protein was decreased (P < 0.05) on days 11–16 of the estrous cycle. The expression of PGR mRNA was higher (P < 0.01) on days 17–20 compared to days 6–10 and 11–16 of the estrous cycle and pregnancy. PGR-A and PGR-B protein levels were elevated on days 1–5 and 17–20 of the estrous cycle as compared to other stages of the cycle and during pregnancy. In conclusion, our results indicate that P4 may influence endometrial cells through both genomic and nongenomic way. This mechanism may contribute to the regulation of the estrous cycle and provide protection during pregnancy. |
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