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Sex-sorting of boar spermatozoa does not influence the localization of glucose transporters
Authors:Diego Bucci  Giovanna Galeati  Elisa Giaretta  Carlo Tamanini  Marcella Spinaci
Affiliation:1. Department of Ichthyology, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 5, 10-719 Olsztyn, Poland;2. Department of Salmonid Research, Inland Fisheries Institute in Olsztyn, Rutki, 83-330 Zukowo, Poland;1. External Quality Assurance Schemes for Reproductive Medicine, PO Box 162, Northlands, WA 6905, Australia;2. Fertility Specialists of Western Australia, Bethesda Hospital, 25 Queenslea Drive, Claremont, WA 6010, Australia;3. Fertility North, Suite 213, Specialist Medical Centre, Joondalup Health Campus, Shenton Avenue, Joondalup, WA 6027, Australia;4. Edith Cowan University, 270 Joondalup Drive, Joondalup, WA 6027, Australia;1. Department of Animal Sciences, University of Illinois, Urbana-Champaign, Illinois, USA;2. United States Department of Agriculture-Agricultural Research Service-National Center for Genetic Resources Preservation-National Animal Germplasm Program, Fort Collins, Colorado, USA;1. Department of Animal Science, Division of Applied Life Science, Graduate School of Gyeongsang National University, Jinju, Gyeongnam, Republic of Korea;2. Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Suwon, Gyeonggi, Republic of Korea;3. Department of Animal Biotechnology, KonKuk University, Seoul, Republic of Korea;4. Department of Animal Science, Pusan National University, Miryang, Gyeongnam, Republic of Korea;5. Department of Veterinary Medicine, Jeju National University, Jeju, Republic of Korea;1. Department of Reproductive Biology and Stem Cells, Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland;2. Institute of Hereditary Pathology, Ukrainian Academy of Medical Sciences, Lysenko Str. 31a, 79000 Lviv, Ukraine;3. Department of Clinical Genetics, Medical University of Bialystok, Waszyngton 13, PO Box 22, 15-089 Bialystok, Poland
Abstract:Sex-sorting damages spermatozoa function, shortening their lifespan and fertility. This study used an immunofluorescence technique to investigate the effect of sex-sorting on the localization of glucose transporters (GLUTs) in boar spermatozoa. GLUTs are trans-membrane proteins responsible for glucose transport within cells. Distribution of GLUTs on sperm cells was similar in unsorted and sex-sorted semen, suggesting that the flow cytometric sex-sorting process did not affect the sperm energy apparatus.
Keywords:GLUT  Sex-sorting  Boar  Spermatozoa  Metabolism
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