Detection of two CSN1S1 variants in Egyptian buffalo |
| |
Authors: | Soheir M. El Nahas Mona A. Bibars Dalia A. Taha Hassan I. El-Sayyad |
| |
Affiliation: | 1. Department of Cell Biology, National Research Center, Giza, Egypt;2. Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt |
| |
Abstract: | The present study investigates CSN1S1 casein gene polymorphism in Egyptian buffalo. CSN1S1 was analyzed in 17 unrelated Egyptian lactating buffalo. The amplified segment includes the last 43 amino acids of Exon 17 and part of Intron 17. In the present study we report for the first time the presence of 2 variants 178Ser (TCA) and 178Leu (TTA) in Egyptian buffalo CSN1S1 gene. The genotypic frequencies in the investigated Egyptian buffalo sample were 0.47, 0.058 and 0.47 for homozygous 178Ser, for homozygous 178Leu and heterozygous 178Leu/Ser, respectively. The 178Ser and 178Leu variant frequencies are 0.64 and 0.36, respectively which indicates the superiority of variant 178Ser in Egyptian buffalo. The allelic frequency in Egyptian buffalo is not much different from the corresponding allelic frequency in Italian buffalo (0.69 and 0.31 for 178Ser and 178Leu, respectively) as reported by Chianese et al. [3]. This is not surprising since they both belong to Mediterranean type. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|