Effects of post-thaw incubation on motility,acrosomal integrity and in vitro fertilizing capacity of boar spermatozoa cryopreserved in 0.5 and 5 ml straws |
| |
| Authors: | Alejandro Córdova Rocío Hernández-Gil Cristian Alejandro Córdova-Jiménez Diogo José Cardilli Kellen de Sousa Oliveira José Félix Pérez-Gutiérrez |
| |
| Institution: | 1. Genética Molecular-Laboratorio Medicina, HUCA, Oviedo, Spain;2. Nefrología, HUCA, Oviedo, Spain;3. Centros de Salud Principado de Asturias, Asturias, Spain;4. Cellular Biology in Renal Diseases Laboratory, IIS-Fundación Jiménez Díaz and Universidad Autónoma de Madrid, Spain;5. Pediatría, HUCA, Oviedo, Spain;6. IDIPAZ, Madrid, Spain;7. Fundación Renal and RED INVESTIGACION RENAL (REDINREN), Spain;8. Universidad de Oviedo, Oviedo, Spain |
| |
| Abstract: | The effect of post-thaw incubation (0 vs. 5 h at 15 °C) and straw size (5 vs. 0.5 ml) on motility, acrosomal integrity and in vitro fertilizing (IVF) capacity of cryopreserved boar spermatozoa was studied. In samples assessed immediately after thawing, no differences were found between the two straw sizes. After 5 h post-thaw incubation, all parameters, except polyspermy, decreased and, spermatozoa packaged in 5 ml straws showed better functional and IVF parameters than these in 0.5 ml straws. |
| |
| Keywords: | Boar Spermatozoa Cryopreservation Post-thaw incubation |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
