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Effect of glucose and phytohaemagglutinin (PHA) rich Phaseolus vulgaris extract on growth and protein synthesis of pharmaceutically important cyanobacteria Nostoc ellipsosporum NCIM 2786
Authors:Srivatsava Anshuman  Mittal Deepika  Govindasamy Sharmila  Chandrasekaran Muthukumaran
Affiliation:Bioprocess Laboratory, Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, Tamilnadu, India
Abstract:Nostoc ellipsosporum is a highly potent cyanobacterium for production of pharmaceutically important chemicals. In this study, an effort has been made to determine the effect of glucose and phytohaemagglutinin (PHA) rich Phaseolus vulgaris extract on N. ellipsosporum growth and protein production. Maximum growth was observed in Fog’s medium supplemented with glucose. SEM analysis showed that the regular and well developed heterocysts were observed in Fog’s media supplemented with glucose. Significant medium components were evaluated by Plackett–Burman (PB) design and PHA extract was found to be the most significant in growth medium. Results of this study showed that both glucose and PHA rich P. vulgaris extract have positive effects and enhance the growth and protein synthesis.
Keywords:Heterocysts  Phytohaemagglutinin  Plackett–Burman
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