The activity of the carbon metabolism enzymes inchromatium minutissimum after long-term storage |
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Authors: | E N KrasH’nikova L M Zakharchuk |
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Institution: | (1) Department of Microbiology,Biological Faculty, Moscow State University, Vorob’evy gory, 119899 Moscow, Russia |
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Abstract: | The activity of the enzymes of the tricarboxylic acid cycle and glyoxylate shunt, as well as of some enzymes involved in carbohydrate
metabolism, were determined in the purple sulfur bacteriumChromatium minutissimum either maintained by subculturing in liquid medium or stored in the lyophilized state for 36 years. In cultures stored in
the lyophilized state, the activities of the key enzymes of the tricarboxylic acid cycle, glyoxylate shunt, and Embden-Meyerhof-Parnas
pathway were higher, whereas the activities of glucoses-phosphate dehydrogenase, pyruvate kinase, and ribulose bisphosphate
carboxylase were somewhat lower than in cultures maintained by regular transfers. |
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Keywords: | purple sulfur bacteria enzymes of the tricarboxylic acid cycle enzymes of carbohydrate metabolism methods for microorganism storage lyophilization |
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