| fliL基因显著影响艰难拟梭菌运动功能及产孢能力 |
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| 引用本文： | 鲍江舰,杨君仪,邵瑞瑞,张婷,廖健,程玉梅,官志忠,齐晓岚,陈峥宏,洪伟,崔古贞.fliL基因显著影响艰难拟梭菌运动功能及产孢能力[J].生物工程学报,2023,39(4):1578-1595. |
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| 作者姓名： | 鲍江舰 杨君仪 邵瑞瑞 张婷 廖健 程玉梅 官志忠 齐晓岚 陈峥宏 洪伟 崔古贞 |
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| 作者单位： | 贵州医科大学基础医学院 贵州省普通高等学校病原生物学特色重点实验室, 贵州 贵阳 550004;贵州医科大学 地方病与少数民族疾病教育部重点实验室 贵州省医学分子生物学重点实验室, 贵州 贵阳 550001;贵州医科大学口腔医学院 附属口腔医院, 贵州 贵阳 550001;贵州医科大学附属医院综合ICU, 贵州 贵阳 550001;贵州医科大学 地方病与少数民族疾病教育部重点实验室 贵州省医学分子生物学重点实验室, 贵州 贵阳 550001;贵州医科大学病理科 贵州医科大学附属医院, 贵州 贵阳 550001;贵州医科大学 地方病与少数民族疾病教育部重点实验室 贵州省医学分子生物学重点实验室, 贵州 贵阳 550001;省部共建地方病及民族区域性疾病防控协同创新中心, 贵州 贵阳 550001 |
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| 基金项目： | 国家自然科学基金(32170134，32160015，U1812403)；贵州省自然科学基金([2020]1Z067，[2019]1441)；贵州医科大学优秀青年人才计划([2022]101) |
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| 摘 要： | 鞭毛基底体相关FliL家族蛋白(flagellar basal body-associated FliL family protein,fliL)基因编码FliL蛋白,FliL是一种与鞭毛基体相结合的单跨膜蛋白。为研究艰难拟梭菌fliL基因功能,使用非等长同源臂偶联等位交换(allele-coupled exchange,ACE)方法成功构建了fliL基因缺失(ΔfliL)和回补(::fliL)突变株,研究突变菌株与野生型菌株(CD630)生长曲线、抗生素敏感性、pH耐受性、运动能力及产孢能力等表型的差异。结果显示,菌株ΔfliL生长速率及最大生物量均小于菌株CD630,::fliL回补菌株生长情况回复至野生型。与CD630菌株相比,ΔfliL对阿莫西林、氨苄青霉素、诺氟沙星的敏感性提高,对卡那霉素、四环素敏感性降低,::fliL抗生素敏感性部分回复至野生型水平。与CD630菌株相比,ΔfliL游泳运动能力显著降低,::fliL运动能力超越野生型菌株CD630。相比菌株CD630,菌株ΔfliL在pH值为5时耐受能力显著提高,在pH值为9时,耐受能力显著降低。除此之外,ΔfliL产孢能力较CD630显著降低,::fliL产孢能力部分恢复。以上结果表明,艰难拟梭菌fliL基因与其运动能力、抗生素敏感性、环境耐受能力和产孢能力密切相关,可能进一步影响艰难拟梭菌菌株的致病力。
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| 关 键 词： | 艰难拟梭菌 非等长同源臂偶联等位交换 鞭毛 fliL 突变株表型 |
| 收稿时间： | 2022/9/24 0:00:00 |
The fliL gene significantly affects the motility and sporulation abilities of Clostridioides difficile |
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| BAO Jiangjian,YANG Junyi,SHAO Ruirui,ZHANG Ting,LIAO Jian,CHENG Yumei,GUAN Zhizhong,QI Xiaolan,CHEN Zhenghong,HONG Wei,CUI Guzhen.The fliL gene significantly affects the motility and sporulation abilities of Clostridioides difficile[J].Chinese Journal of Biotechnology,2023,39(4):1578-1595. |
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| Authors: | BAO Jiangjian YANG Junyi SHAO Ruirui ZHANG Ting LIAO Jian CHENG Yumei GUAN Zhizhong QI Xiaolan CHEN Zhenghong HONG Wei CUI Guzhen |
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| Institution: | Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou, School of Basic Medical Science, Guizhou Medical University, Guiyang 550004, Guizhou, China;Key Laboratory of Medical Molecular Biology of Guizhou Province, Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang 550001, Guizhou, China;Affiliated Stomatological Hospital, School of Stomatology, Guizhou Medical University, Guiyang 550001, Guizhou, China;Department of Critical Care Medicine, the Affiliated Hospital of Guizhou Medical University, Guiyang 550001, Guizhou, China;Key Laboratory of Medical Molecular Biology of Guizhou Province, Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang 550001, Guizhou, China;The Affiliated Hospital of Guizhou Medical University, Department of Pathology, Guizhou Medical University, Guiyang 550001, Guizhou, China;Key Laboratory of Medical Molecular Biology of Guizhou Province, Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang 550001, Guizhou, China;Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-Constructed by the Province and Ministry, Guiyang 550001, Guizhou, China |
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| Abstract: | |
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| Keywords: | Clostridioides difficile allele-coupled exchange (ACE) flagellum fliL phenotypes of mutants |
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