Effects of Postmortem Interval on Mouse Ovary Oocyte Survival and Maturation |
| |
Authors: | Guang-Li Zhang Jun-Yu Ma Quan Sun Meng-Wen Hu Xiu-yan Yang Si-Hua Gao Guang-Jian Jiang |
| |
Affiliation: | 1. Diabetes research Center of Beijing University of Chinese Medicine, Beijing, China.; 2. Department of Biochemistry and Molecular Biology, Hebei United University, Tangshan, China.; 3. Department of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.; 4. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.; Institute of Zoology, Chinese Academy of Sciences, China, |
| |
Abstract: | To study the time- and temperature-dependent survival of ovarian oocytes collected from postmortem carcass, ICR mice were killed and placed for different periods (0, 1, 2, 4, 6, 8 and 10 h) at different temperatures (25°C, 4°C and 37°C). After preservation, oocyte morphology, germinal vesicle (GV) oocyte number, oocyte meiotic maturation percentage, mitochondrial distribution and intracellular glutathione (GSH) level were evaluated. The results showed no surviving oocytes could be collected by 2h, 6h, and 12 h after carcass preservation at 37°C, 25°C and 4°C, respectively. The number of collected GV oocytes in the ovary deceased as the preservation time lasted at the same temperature. Meanwhile at the same point in time, the ratio of germinal vesicle breakdown (GVBD) and the first polar body emission (PBE) gradually reduced as preservation temperature increased. In addition, the percentage of abnormal mitochondrial distribution in the preserved oocytes was obviously higher than that in the control oocytes, while GSH level was not altered in collected oocytes. Unexpectedly, neither chromosome arrangement nor spindle organization was affected as long as the oocytes from preserved carcasses could complete maturation. These data are helpful for proper use of ovary oocytes from postmortem carcass of valuable individuals. |
| |
Keywords: | |
|
|