Flower color alteration in <Emphasis Type="Italic">Lotus japonicus</Emphasis> by modification of the carotenoid biosynthetic pathway |
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Authors: | Sakae Suzuki Masahiro Nishihara Takashi Nakatsuka Norihiko Misawa Isao Ogiwara Saburo Yamamura |
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Institution: | (1) Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate 024-0003, Japan;(2) Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan;(3) Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan |
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Abstract: | To establish a model system for alteration of flower color by carotenoid pigments, we modified the carotenoid biosynthesis
pathway of Lotus japonicus using overexpression of the crtW gene isolated from marine bacteria Agrobacterium aurantiacum and encoding β-carotene ketolase (4,4′-β-oxygenase) for the production of pink to red color ketocarotenoids. The crtW gene with the transit peptide sequence of the pea Rubisco small subunit under the regulation of the CaMV35S promoter was
introduced to L. japonicus. In most of the resulting transgenic plants, the color of flower petals changed from original light yellow to deep yellow
or orange while otherwise exhibiting normal phenotype. HPLC and TLC analyses revealed that leaves and flower petals of these
plants accumulated novel carotenoids, believed to be ketocarotenoids consisting of including astaxanthin, adonixanthin, canthaxanthin
and echinenone. Results indicated that modification of the carotenoid biosynthesis pathway is a means of altering flower color
in ornamental crops. |
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Keywords: | Carotenoid Flower color Ketocarotenoid Lotus japonicus Transit peptide |
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