Characterization of two possible forms of type IV collagen from human kidney cortex |
| |
Authors: | T Oikawa K Sayama Y Matsuda Y Fujimoto T Iwaguchi A Matsuzawa |
| |
Affiliation: | Division of Cancer Therapeutics, Tokyo Metropolitan Institute of Medical Science, Japan. |
| |
Abstract: | Type IV collagen was isolated from human kidney cortex according to the purification of mouse kidney type IV collagen (MKIVC) with minor modifications as described previously [Oikawa, T., et al. (1986) Chem. Pharm. Bull. 34, 789-797]. Chromatography of human kidney type IV collagen (HKIVC) on a column of DEAE-cellulose resulted in its separation into two fractions, i.e., one (HKIVC-1) passed through and the other (HKIVC-2) adsorbed to the column, similar to MKIVC. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Bio-Gel A-5m chromatography revealed that both HKIVC-1 and -2 differed from each other in the ratios of major and minor components. Amino acid analyses also demonstrated that there was a significant difference in the contents of several amino acid residues between both type IV collagens and their 80-kDa components, one of the major ones. These results indicate the possibility that there exist two forms of type IV collagen in human kidney. |
| |
Keywords: | |
|
|